CD4 Protein (CD4) (AA 24-216) (His tag,Strep II tag)

N° du produit ABIN2468318

Détail du produit

Antigène: CD4

Type de proteíne: Recombinant

Attributs du protein: AA 24-216

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CD4 protéine est marqué à la His tag,Strep II tag.

Application: Western Blotting (WB), ELISA, Mass Spectrometry (MS), Neutralization (Neut)

Pureté: ~90 %

Information d'application

Indications d'application: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM sodium phosphate, 500 mM NaCl, 25 mM imidazole, 10 % gycerol

Conseil sur la manipulation: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -80 °C

Stockage commentaire: Store in working aliquots at -70 °C.

Détail du antigène

Antigène: CD4

Autre désignation: CD4 (CD4 Produits)

Synonymes: cd4 Protein, L3T4 Protein, Ly-4 Protein, CD4mut Protein, W3/25 Protein, p55 Protein, CD4 molecule Protein, carcinoembryonic antigen-related cell adhesion molecule 5 Protein, T-cell surface glycoprotein CD4 Protein, CD4 antigen Protein, Cd4 molecule Protein, CD4 Protein, LOC100125525 Protein, cd4 Protein, Cd4 Protein

Sujet: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response (1). On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 Molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120 (2). This soluble CD4 fragment (sCD4) will work in HIV neutralization assays at levels comparable to previously published results. The minor upper band seen in the Coomassie blue-stained gel is likely to be due to the presence of uncleaved signal sequence.

Poids moléculaire: 27 kDa (Calculated)

ID gène: 920

NCBI Accession: NP_000607

OMIM: 10835167

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events