AKT1 Protéine

N° du produit ABIN5624567

N° du produit (Fournisseur): 009-001-p21

Cette protéine Recombinant AKT1 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour AKT1 Protéine (ABIN5624567)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Détail du produit

N° du produit (Fournisseur): 009-001-p21

Fournisseur: Rockland

Fonction: AKT1 Human Recombinant Protein

Purification: Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1, Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Information d'application

Indications d'application:

Application Note: Human AKT1 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.16 mg/mL

Buffer:

Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

Stabilizer: 10 % (v/v) Glycerol

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20°C or colder prior to use. Avoid cycles of freezing and thawing, aliquot and freeze at -20° C or colder. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) .

Date de péremption: 12 months

Détail du antigène

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1 Protein

Sujet:

Synonyms: RAC, PKB, AKT, PKB alpha, PRKBA, activated AKT

Background: AKT1 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. AKT1 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

ID gène: 207, 62241011

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg