Insulin Protein (INS) (AA 25-54, AA 90-110) (His tag)

N° du produit ABIN6239790

Détail du produit

Antigène: Insulin (INS)

Activité biologique: Active

Attributs du protein: AA 25-54, AA 90-110

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette Insulin protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: INS (Insulin) is a peptide hormone produced by beta cells of the pancreatic islets, which decreases blood glucose concentration and increases cell permeability to monosaccharides, amino acids and fatty acids. It has been reported that insulin triggers phosphorylation of a number of substrates by binding to its receptors, which was important for cell proliferation, cell cycle progression, cell division and differentiation. To detect the effect of Insulin on cell proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of INS. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C.

Information d'application

Indications d'application: Isoelectric Point: 7.1

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: Insulin (INS)

Abstract: INS Produits

Synonymes: IDDM2 Protein, ILPR Protein, IRDN Protein, MODY10 Protein, ins1 Protein, xins Protein, ins1-a Protein, Insulin Protein, AA986540 Protein, Ins-2 Protein, InsII Protein, Mody Protein, Mody4 Protein, proinsulin Protein, zgc:109842 Protein, igf2-A Protein, ins Protein, ins-a Protein, ins-b Protein, insulin Protein, insulin precursor Protein, insulin II Protein, preproinsulin Protein, insulin L homeolog Protein, insulin S homeolog Protein, INS Protein, INS-IGF2 Protein, ins Protein, Ins Protein, PIN Protein, Ins2 Protein, ins.L Protein, ins.S Protein

Sujet: Alternative Names: Insulin

Poids moléculaire: 10kDa

UniProt: P01315

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation