ITGAV/ITGB8 Protein (AA 31-992) (His tag,AVI tag,Biotin)

N° du produit ABIN6253195

Détail du produit

Antigène: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Type de proteíne: Recombinant

Attributs du protein: AA 31-992

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ITGAV/ITGB8 protéine est marqué à la His tag,AVI tag,Biotin.

Séquence: AA 31-992

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Pureté: >90 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 8.0

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Autre désignation: Integrin alpha V beta 8 (ITGAV/ITGB8 Produits)

Sujet: Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

Poids moléculaire: 114.7 kDa (ITGAV) & 76.5 kDa (ITGB8)