MASP2 Protein (AA 287-686) (His tag)

N° du produit ABIN7275291

Détail du produit

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Type de proteíne: Recombinant

Attributs du protein: AA 287-686

Origine: Cynomolgus

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MASP2 protéine est marqué à la His tag.

Fonction: Cynomolgus MASP2 Protein

Séquence: Thr287-Phe686

Attributs du produit: Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686.

Pureté: > 95 % as determined by Tris-Bis PAGE

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Autre désignation: MASP2 (MASP2 Produits)

Synonymes: MAP19 Protein, MASP-2 Protein, MASP1P1 Protein, sMAP Protein, MAp19 Protein, mannan binding lectin serine peptidase 2 Protein, mannan-binding lectin serine peptidase 2 Protein, MASP2 Protein, Masp2 Protein

Sujet: The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.

Poids moléculaire: 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5UJY0

Pathways: Système du Complément