MASP2 Protein (AA 288-686) (His tag)

N° du produit ABIN7275292

Cette protéine Recombinant MASP2 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MASP2 Protein (AA 288-686) (His tag) (ABIN7275292)

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Tris-Bis PAGE

Détail du produit

Attributs du protein: AA 288-686

Purification/Conjugué: Cette MASP2 protéine est marqué à la His tag.

Fonction: Human MASP2 Protein

Séquence: Gly288-Phe686

Attributs du produit: Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MASP2, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Autre désignation: MASP2

Sujet: The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.

Poids moléculaire: 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.

UniProt: O00187-1

Pathways: Système du Complément