MASP2 Protein (AA 287-685) (His tag)

N° du produit ABIN7275294

Cette protéine Recombinant MASP2 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour MASP2 Protein (AA 287-685) (His tag) (ABIN7275294)

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Type de proteíne: Recombinant

Origine: Rat

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by Tris-Bis PAGE

Détail du produit

Attributs du protein: AA 287-685

Purification/Conjugué: Cette MASP2 protéine est marqué à la His tag.

Fonction: Rat MASP2 Protein

Séquence: Thr287-Phe685

Attributs du produit: Recombinant Rat MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe685.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Rat MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))

Autre désignation: MASP2

Sujet: The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.

Poids moléculaire: 46.7 kDa. Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result.

UniProt: A2VCV7

Pathways: Système du Complément