TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
Type de proteíne
Recombinant
Attributs du protein
AA 30-211
Origine
Humain
Source
HEK-293 Cells
Purification/Conjugué
Cette TNFRSF1A protéine est marqué à la Fc Tag.
Fonction
Human TNFR1/CD120a/TNFRSF1A Protein
Séquence
Leu30-Thr211
Attributs du produit
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu30-Thr211.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA. See testing image for detail.
TNFRSF1A
Origine: Souris
Hôte: HEK-293 Cells
Recombinant
> 96 % as determined by SDS-PAGE
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
Poids moléculaire
48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.