Recombinant Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A).
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
IL12
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Poids moléculaire
34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Tris-Bis PAGE result.