anti-Humain MGMT anticorps pour Simple Western

Recommended MGMT Antibody (fourni par: Connectez-vous pour afficher )

Antigène
O6-Methylguanine-DNA-Methyltransferase (MGMT) Anticorps
  • AGT
  • AI267024
  • Agat
  • O-6-methylguanine-DNA methyltransferase
  • MGMT
  • Mgmt
Reactivité
Humain
208
38
24
5
3
2
2
2
2
1
1
1
Hôte
Souris
126
89
1
Clonalité
Monoclonal
Conjugué
Cet anticorp MGMT est non-conjugé
7
7
6
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
191
91
62
56
47
45
38
11
9
2
1
1
1
1
Fournisseur
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N° du produit (Fournisseur)
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Images

N° du produit ABIN151202
Produit non disponible pour cette région.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Fournisseur Clonality References Details
1 ABIN4334172 FACS ICC IF IHC IHC (p) SimWes WB PerCP Mouse IgG2b Connectez-vous pour afficher MT 23-2 0

General

Antigène O6-Methylguanine-DNA-Methyltransferase (MGMT) Anticorps
Reactivité Humain
(208), (38), (24), (5), (3), (2), (2), (2), (2), (1), (1), (1)
Hôte Souris
(126), (89), (1)
Clonalité (Clone)
Monoclonal   ( )
Conjugué Cet anticorp MGMT est non-conjugé
(7), (7), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(191), (91), (62), (56), (47), (45), (38), (11), (9), (2), (1), (1), (1), (1)
Pubmed 21 références disponible
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Détail du produit anti-MGMT anticorps

Détail du antigène MGMT Information d'application Stockage References for anti-MGMT antibody (ABIN151202) Images
Aucune reactivité croisée Souris
Purification Protein G purified
Immunogène Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
Clone MT 23-2
Isotype IgG2b
Plasmids, Primers & others

Détail du antigène MGMT

Détail du produit anti-MGMT anticorps Information d'application Stockage References for anti-MGMT antibody (ABIN151202) Images Haut de la page
Antigène
Autre désignation MGMT (MGMT Antibody Extrait)
Sujet Gene Symbol: MGMT
Poids moléculaire Theoretical MW: 24 kDa
ID gène 4255
UniProt P16455
Pathways Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus

Information d'application

Détail du produit anti-MGMT anticorps Détail du antigène MGMT Stockage References for anti-MGMT antibody (ABIN151202) Images Haut de la page
Indications d'application Western Blot 1:1000, Simple Western 1:1000, Flow Cytometry 1-2 μg/mL, Immunohistochemistry 1:500-1000, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-Paraffin 1:500-1:1000, Flow (Intracellular), CyTOF-readyThis MGMT (MT 23.2) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, a band at approx. 24 kDa can be seen. In ICC/IF, strong staining has been seen in CEM cells and no signal has been seen in TK6 cells. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Protocol specific for MGMT Antibody Procedure Guide for NB 100-168 Monoclonal anti-MGMTWestern Blot Procedure
1. Run ~ 50 _g of whole cell extract on a PAGE gel (ie: 4-12 % NuPAGE Bis-Tris gel).
. Transfer the protein from the gel to a nitrocellulose membrane for 45-60 minutes.
. Block the membrane with blocking buffer [1X TBS / 0.1 % Tween-20 / 5 % NFDM] for 1 hour at room temperature.
. Rinse the membrane with 1X TBS, twice.
. Dilute the 1_ antibody (cat# NB100-168, anti-MGMT) 1:1,000 to 1:3,000, in dilution buffer [1X TBS + 1 % BSA].
. Incubate the membrane with 1_ antibody for 1 hour at room temperature.
. Wash the membrane with washing buffer [1X TBS + 0.05 % Tween-20] 1 X 15 minutes, 3 x 5 minutes.
. Dilute 2_ antibody [anti-mouse-HRP] in dilution buffer, add to the membrane, and incubate for 35 minutes at room temperature.
. Wash the membrane with washing buffer 1 X 15 minutes, 3 x 5 minutes.
. Develop the membrane with an ECL kit (ie: ChemiGlow Chemiluminescence reagent from AlphaInnotech].
Restrictions For Research Use only

Stockage

Détail du produit anti-MGMT anticorps Détail du antigène MGMT Information d'application References for anti-MGMT antibody (ABIN151202) Images Haut de la page
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-MGMT antibody (ABIN151202)

Détail du produit anti-MGMT anticorps Détail du antigène MGMT Information d'application Stockage Images Haut de la page
Produit citée dans:

Kotsarenko, Lylo, Ruban, Macewicz, Lukash: "Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines." dans: Biochemical genetics, Vol. 56, Issue 5, pp. 459-477, 2018

Altinoz, Elmaci, Bolukbasi, Ekmekci, Yenmis, Sari, Sav: "MGMT gene variants, temozolomide myelotoxicity and glioma risk. A concise literature survey including an illustrative case." dans: Journal of chemotherapy (Florence, Italy), Vol. 29, Issue 4, pp. 238-244, 2017 (Échantillon (espèces): Human).

Oktay, Ülgen, Can, Akyerli, Yüksel, Erdemgil, Durası, Henegariu, Nanni, Selevsek, Grossmann, Erson-Omay, Bai, Gupta, Lee, Turcan, Özpınar, Huse, Sav, Flanagan, Günel, Sezerman, Yakıcıer, Pamir et al.: "IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. ..." dans: Scientific reports, Vol. 6, pp. 27569, 2016 Détails: Immunohistochemistry

Stepanenko, Andreieva, Korets, Mykytenko, Baklaushev, Huleyuk, Kovalova, Kotsarenko, Chekhonin, Vassetzky, Avdieiev, Dmitrenko: "Temozolomide promotes genomic and phenotypic changes in glioblastoma cells." dans: Cancer cell international, Vol. 16, pp. 36, 2016

Fang, Inanc, Schamus, Wang, Wei, Brown, Svilar, Sugrue, Goellner, Zeng, Yates, Lan, Vens, Sobol: "HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β." dans: Nature communications, Vol. 5, pp. 5513, 2014 Détails: Western Blotting

Herzig, Hildreth, Huamani, Perez, Goins, McMahan, Reddick, Walter: "Human O6 -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice." dans: Molecular carcinogenesis, Vol. 52, Issue 4, pp. 275-85, 2013 (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Kewitz, Stiefel, Kramm, Staege: "Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells." dans: Leukemia research, Vol. 38, Issue 1, pp. 138-43, 2013 (Échantillon (espèces): Human). Détails: Western Blotting

Lylo, Matsevich, Kotsarenko, Babenko, Korneliuk, Sukhorada, Lukash: "[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]." dans: T͡Sitologii͡a i genetika, Vol. 45, Issue 6, pp. 53-60, 2012

Persano, Pistollato, Rampazzo, Della Puppa, Abbadi, Frasson, Volpin, Indraccolo, Scienza, Basso: "BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1α stability and MGMT expression." dans: Cell death & disease, Vol. 3, pp. e412, 2012 (Échantillon (espèces): Human). Détails: Western Blotting

Tang, Svilar, Trivedi, Wang, Goellner, Moore, Hamilton, Banze, Brown, Sobol: "N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide." dans: Neuro-oncology, Vol. 13, Issue 5, pp. 471-86, 2011 (Échantillon (espèces): Human). Détails: Western Blotting

Della Puppa, Persano, Masi, Rampazzo, Sinigaglia, Pistollato, Denaro, Barzon, Palù, Basso, Scienza, dAvella: "MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?" dans: Journal of neuro-oncology, Vol. 106, Issue 1, pp. 33-41, 2011

Pistollato, Abbadi, Rampazzo, Persano, Della Puppa, Frasson, Sarto, Scienza, Davella, Basso: "Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma." dans: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 851-62, 2010 (Échantillon (espèces): Human). Détails: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Cai, Xu, Cooper, Ferkowicz, Hartwell, Pollok, Kelley: "Mitochondrial targeting of human O6-methylguanine DNA methyltransferase protects against cell killing by chemotherapeutic alkylating agents." dans: Cancer research, Vol. 65, Issue 8, pp. 3319-27, 2005 Détails: Western Blotting

Zuo, Ai, Ratliff, Suen, Hanna, Brent, Fan: "O6-methylguanine-DNA methyltransferase gene: epigenetic silencing and prognostic value in head and neck squamous cell carcinoma." dans: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 13, Issue 6, pp. 967-75, 2004 (Échantillon (espèces): Human). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Roth, Xu, Luo, Kelley: "Human-yeast chimeric repair protein protects mammalian cells against alkylating agents: enhancement of MGMT protection." dans: Cancer gene therapy, Vol. 10, Issue 8, pp. 603-10, 2003 Détails: Western Blotting

Liu, Spiro, Qin, Majka, Haaga, Schupp, Willson, Gerson: "Differential degradation rates of inactivated alkyltransferase in blood mononuclear cells and tumors of patients after treatment with O(6)-benzylguanine." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 8, pp. 2318-24, 2001 (Échantillon (espèces): Human). Détails: Immunohistochemistry

Background publications

Houghton, Stewart, Cheshire, Richmond, Kirstein, Poquette, Tan, Friedman, Brent: "Antitumor activity of temozolomide combined with irinotecan is partly independent of O6-methylguanine-DNA methyltransferase and mismatch repair phenotypes in xenograft models." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 6, Issue 10, pp. 4110-8, 2001

Sawai, Zhou, Vanin, Houghton, Brent, Sorrentino: "Protection and in vivo selection of hematopoietic stem cells using temozolomide, O6-benzylguanine, and an alkyltransferase-expressing retroviral vector." dans: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 3, Issue 1, pp. 78-87, 2001

Esteller, Corn, Baylin, Herman: "A gene hypermethylation profile of human cancer." dans: Cancer research, Vol. 61, Issue 8, pp. 3225-9, 2001

Herfarth, Brent, Danam, Remack, Kodner, Wells, Goodfellow: "A specific CpG methylation pattern of the MGMT promoter region associated with reduced MGMT expression in primary colorectal cancers." dans: Molecular carcinogenesis, Vol. 24, Issue 2, pp. 90-8, 1999

Images

Détail du produit anti-MGMT anticorps Détail du antigène MGMT Information d'application Stockage References for anti-MGMT antibody (ABIN151202) Haut de la page
Images (Fournisseur)
Western Blotting (WB) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Detection of MGMT in CEM whole cell extract using ABIN151202 at 1:100 dilution.
Simple Western (SimWes) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Simple Western: MGMT Antibody (MT 23.2) [ABIN151202] - Simple Western lane view shows...
Western Blotting (WB) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Western Blot: MGMT Antibody (MT 23.2) [ABIN151202] - Analysis of MGMT (MT 23.2) antib...
 image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Flow (Intracellular): MGMT Antibody (MT 23.2) [ABIN151202] - An intracellular stain w...
 image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Flow (Intracellular): MGMT Antibody (MT 23.2) [ABIN151202] - An intracellular stain w...
Immunofluorescence (IF) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [ABIN151202] - Detect...
Immunofluorescence (IF) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [ABIN151202] - MCF7 c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Immunohistochemistry-Paraffin: MGMT Antibody (MT 23.2) - IHC analysis of a formalin ...
Flow Cytometry (FACS) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody (ABIN151202) Flow Cytometry: MGMT Antibody (MT 23.2) - An intracellular stain was performed on He...