AKT1 anticorps (pThr308)
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- Antigène Voir toutes AKT1 Anticorps
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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Épitope
- AA 302-311, pThr308
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp AKT1 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Séquence
- DGATMK(p)TFCG
- Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated Human AKT (pThr308). Minimal reactivity occurs against non-phosphorylated AKT.
- Purification
- Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
- Immunogène
- Synthetic peptide corresponding to aa 302-311 of Human AKT protein conjugated to KLH
- Top Product
- Discover our top product AKT1 Anticorps primaire
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- Indications d'application
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ELISA (1/15,000). Western blotting (1/500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.97 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- Autre désignation
- AKT1 / PKB (AKT1 Produits)
- Synonymes
- anticorps AKT, anticorps CWS6, anticorps PKB, anticorps PKB-ALPHA, anticorps PRKBA, anticorps RAC, anticorps RAC-ALPHA, anticorps Akt, anticorps Rac, anticorps PKB/Akt, anticorps PKBalpha, anticorps AKT/PKB, anticorps AKT1, anticorps Akt/PKB, anticorps Akt1, anticorps CG4006, anticorps D-Akt, anticorps DAKT1, anticorps DAKT1/PKB, anticorps DAkt, anticorps DAkt1, anticorps DPKB, anticorps DRAC-PK, anticorps DRAC-PK66, anticorps DRAC-PK85, anticorps Dakt, anticorps Dakt1, anticorps Dmel\\CG4006, anticorps Dpkb, anticorps PKB/AKT, anticorps PKB/dAKT, anticorps RacPK, anticorps akt, anticorps akt1, anticorps dAKT, anticorps dAKT/dPKB, anticorps dAKT1, anticorps dAkt, anticorps dAkt/PKB, anticorps dAkt1, anticorps dPKB, anticorps dakt, anticorps dakt1, anticorps l(3)04226, anticorps l(3)89Bq, anticorps p-Akt, anticorps pAkt, anticorps ACT-5, anticorps akt-1, anticorps pkb, anticorps v-akt, anticorps v-akt1, anticorps xAct, anticorps ATAKT1, anticorps F18A8.2, anticorps F18A8_2, anticorps K+ transporter 1, anticorps POTASSIUM TRANSPORTER, anticorps AKT serine/threonine kinase 1, anticorps thymoma viral proto-oncogene 1, anticorps CG4006 gene product from transcript CG4006-RE, anticorps actin beta, anticorps v-akt murine thymoma viral oncogene homolog 1 S homeolog, anticorps v-akt murine thymoma viral oncogene homolog 1, anticorps K+ transporter 1, anticorps Serine/threonine-protein kinase akt-1, anticorps AKT1, anticorps Akt1, anticorps ACTB, anticorps akt1.S, anticorps akt1, anticorps KT1, anticorps akt-1
- Sujet
- AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
- ID gène
- 207
- NCBI Accession
- NP_001014431
- UniProt
- P31749
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg
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