E-cadherin anticorps
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- Antigène Voir toutes E-cadherin (CDH1) Anticorps
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp E-cadherin est non-conjugé
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purification
- Protein A chromatography
- Immunogène
- Breast carcinoma cell line T47D
- Clone
- 67A4-2-1
- Isotype
- IgG1
- Top Product
- Discover our top product CDH1 Anticorps primaire
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- Indications d'application
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Suitable for Immunohistochemistry (frozen sections: 4-8 μg/mL, 1/50-1: 100) and FACS. Suggested positive control: Human tonsil. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water. For best results, dilute in TBS or other phosphate-free buffer containing 10 mM Ca2+.
- Concentration
- 0.4 mg/mL
- Buffer
- PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.09 % sodium azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
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- Antigène
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- Autre désignation
- CD324 / Cadherin-1 (CDH1 Produits)
- Synonymes
- anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S
- Sujet
- E-Cadherin, also called L-CAM (Liver Cell Adhesion Molecule) is an epithelial cell adhesion molecule and plays an important role in the formation of cell-cell contacts in epithelia irrespective of their origin as ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex and is as such important in maintaining epithelial integrity. Over 90 % of malignancies are carcinomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-cadherin expression. Therefore, the expression of E-cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin
- ID gène
- 999
- NCBI Accession
- NP_004351
- UniProt
- P12830
- Pathways
- Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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