E-cadherin anticorps

N° du produit ABIN112362

Détail du produit anti-E-cadherin anticorps

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E-cadherin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: MB2 recognizes both the 120 kDa E-cadherin and its 80 kDa trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion.

Purification: Purified

Immunogène: MB2 is a mouse monoclonal IgG2b antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with MCF- 7/AZ cells expressing E- cadherin at their cell surface.

Clone: MB2

Isotype: IgG2b

Information d'application

Indications d'application: MB2 is useful for Flow cytometry, Immunoblotting, Immunocytochemistry on fixed cells(methanol fixation) and Immunohistochemistry on frozen tissues when using a PBS buffercontaining 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended dilutions: Flow cytometry 1/100-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/100-1/200.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store lyophilized antibody at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for up to two weeks. For longer storage aliquot and store frozen at -20 °C to -80 °C.

Détails sur E-cadherin

Antigène: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Autre désignation: CD324 / Cadherin-1 (CDH1 Produits)

Synonymes: anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S

Sujet: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin αEβ7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently down-regulated, which is consistent with its function of an invasion suppressor in normal epithelia.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin

ID gène: 999

NCBI Accession: NP_004351

UniProt: P12830

Pathways: Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation