FYN anticorps (AA 7-176)

N° du produit ABIN120706

Détail du produit anti-FYN anticorps

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Épitope: AA 7-176

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FYN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Specificité: The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (~59 kDa), which belongs to the Src family of cytoplasmic tyrosine kinases.

Purification: Protein A Affinity Chromatography

Immunogène: Bacterially expressed recombinant fragment of human Fyn (aa 7-176)

Clone: FYN-01

Isotype: IgG2b

Information d'application

Indications d'application: Immunoprecipitation (Positive control: J77 T cell line, Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1 % laurylmaltosid (w/v), 50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.) Western Blotting (2 mg/mL, Positive control: JURKAT human T cell leukemia cell line). Immunocytochemistry. Immunohistochemistry (paraffin sections): 5 m g/mL. Positive tissue: brain
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Date de péremption: 12 months

Détails sur FYN

Antigène: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Autre désignation: FYN (FYN Produits)

Synonymes: anticorps im:6908851, anticorps si:dkey-119m7.5, anticorps zgc:109996, anticorps c-fyn, anticorps cfyn-a, anticorps SLK, anticorps SYN, anticorps p59-FYN, anticorps PKC, anticorps fyn, anticorps zgc:86720, anticorps AI448320, anticorps AW552119, anticorps FYN proto-oncogene, Src family tyrosine kinase, anticorps FYN proto-oncogene, Src family tyrosine kinase S homeolog, anticorps FYN proto-oncogene, Src family tyrosine kinase b, anticorps FYN proto-oncogene, Src family tyrosine kinase L homeolog, anticorps FYN proto-oncogene, Src family tyrosine kinase a, anticorps Fyn proto-oncogene, anticorps FYN, anticorps fyn.S, anticorps fynb, anticorps fyn.L, anticorps Fyn, anticorps fyna

Sujet: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

ID gène: 2534

UniProt: P06241

Pathways: Signalistation JAK/STAT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling