SHC1 anticorps (N-Term)
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- Antigène Voir toutes SHC1 Anticorps
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp SHC1 est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- This antibody specifically recognizes the N- terminus of Shc/p66.
- Attributs du produit
- Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1
- Purification
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Synthetic peptide conjugated to KLH
- Clone
- 2-40E-005
- Isotype
- IgG1
- Top Product
- Discover our top product SHC1 Anticorps primaire
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- Indications d'application
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated Neuro 2A cells (See included Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Included Positive Control: Cell lysate from untreated Neuro 2A cells
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.09 % Sodium-Azide, PEG and Sucrose, 50 % Glycerol
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- Autre désignation
- SHC1 / SHC (SHC1 Produits)
- Synonymes
- anticorps SHC1, anticorps SHC, anticorps SHCA, anticorps P66shc, anticorps si:dkey-246i14.4, anticorps wu:fc10h03, anticorps shc, anticorps p66shc, anticorps Shc, anticorps ShcA, anticorps p66, anticorps SHC adaptor protein 1, anticorps Shc1p, anticorps squalene--hopene cyclase, anticorps SHC (Src homology 2 domain containing) transforming protein 1, anticorps SHC (Src homology 2 domain containing) transforming protein 1 L homeolog, anticorps src homology 2 domain-containing transforming protein C1, anticorps SHC1, anticorps shc1, anticorps Shc1, anticorps shc1.L
- Sujet
- Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1
- Poids moléculaire
- 66 kDa
- ID gène
- 6464
- UniProt
- P29353
- Pathways
- Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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