CHEK1 anticorps (Ser280)

N° du produit ABIN1574028

Détail du produit anti-CHEK1 anticorps

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Épitope: Ser280

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody detects endogenous levels of human Chk1 protein.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide (KLH-coupled) derived from AA surrounding Ser280 of human Chk1

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur CHEK1

Antigène: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Autre désignation: Chk1 (CHEK1 Produits)

Synonymes: anticorps An08g10320, anticorps AO090003000441, anticorps CHK1, anticorps chk1, anticorps C85740, anticorps Chk1, anticorps rad27, anticorps id:ibd2720, anticorps zgc:56093, anticorps CHEK1, anticorps checkpoint kinase 1, anticorps serine/threonine-protein kinase chk1, anticorps serine/threonine-protein kinase Chk1, anticorps CAMK/CAMKL/CHK1 protein kinase Chk1, anticorps checkpoint kinase 1 S homeolog, anticorps CHEK1, anticorps ANI_1_2436074, anticorps AOR_1_768154, anticorps PTRG_04183, anticorps SJAG_01680, anticorps PAAG_04978, anticorps MCYG_03290, anticorps VDBG_03742, anticorps chek1.S, anticorps Chek1, anticorps chek1

Sujet: Chk1 is a serine/threonine kinase first identified as a checkpoint kinase in Schizosaccharomyces pombe. Chk1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. Chk1 is activated through phosphorylation by ATR/ATM and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. The activation of Chk1 in response to DNA replication blockages and certain forms of genotoxic stress involves phosphorylation of Ser317 and Ser345. Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 further activates downstream factors that mediate cell cycle arrest and other cell physiological events through phosphorylation. These key cell cycle factors include p53, Cdc25C and more. Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide derived from the residues surrounding Ser 280 of human Chk1 (Swiss Prot: O14757).

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire, Réparation de l'ADN