MDM2 anticorps (AA 154-167)
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- Antigène Voir toutes MDM2 Anticorps
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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Épitope
- AA 154-167
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MDM2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
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This antibody recognizes the 90 kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition. May also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
Cellular Localization: nuclear. - Attributs du produit
- Synonyms: p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2, E3ubiquitin-protein ligase Mdm2
- Purification
- Affinity Chromatography on Protein G.
- Immunogène
- A Synthetic peptide corresponding to amino acids 154-167 of human MDM2 protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8. 653myeloma cell line. AA Sequence: Cys-SRPSTSSRRRAISE
- Clone
- SMP14
- Isotype
- IgG1
- Top Product
- Discover our top product MDM2 Anticorps primaire
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- Indications d'application
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Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/500. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections. EDTA pH 8.0 isrecommended for this purpose. Recommended Positive Control: Breast Carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Autre désignation
- MDM2 (MDM2 Produits)
- Synonymes
- anticorps ACTFS, anticorps HDMX, anticorps hdm2, anticorps zgc:109834, anticorps 1700007J15Rik, anticorps AA415488, anticorps Mdm-2, anticorps MDM2 proto-oncogene, anticorps MDM2 proto-oncogene S homeolog, anticorps Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse), anticorps MDM2 oncogene, E3 ubiquitin protein ligase, anticorps transformed mouse 3T3 cell double minute 2, anticorps MDM2, anticorps mdm2.S, anticorps Mdm2, anticorps mdm2
- Sujet
- Human homolog of Murine Double Minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent, in a wide variety of malignancies of bladder, breast, testes, cervix, and glial cells.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
- ID gène
- 4193
- UniProt
- Q00987
- Pathways
- Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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