AKT2 anticorps

N° du produit ABIN1892264

Détail du produit anti-AKT2 anticorps

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88 % identity to mouse and 90 % identity with rat, therefore, cross reactivity is expected.

Purification: Protein A purified

Stérilité: Sterile filtered

Immunogène: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Type of Immunogen: Synthetic peptide

Clone: 11F6-B2

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:10000), IHC (20 μg/mL), WB (1:500 - 1:2000)

Usage: Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, sterile filtered

Agent conservateur: Azide free

Conseil sur la manipulation: avoid freeze thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Détails sur AKT2

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Autre désignation: AKT2 (AKT2 Produits)

Synonymes: anticorps AKT2, anticorps pkbb, anticorps pkbbeta, anticorps pkbss, anticorps prkbb, anticorps rac-beta, anticorps v-akt2, anticorps 2410016A19Rik, anticorps AW554154, anticorps PKB, anticorps PKBbeta, anticorps HIHGHH, anticorps PKBB, anticorps PKBBETA, anticorps PRKBB, anticorps RAC-BETA, anticorps cb945, anticorps akt2-a, anticorps AKT serine/threonine kinase 2, anticorps v-akt murine thymoma viral oncogene homolog 2, anticorps thymoma viral proto-oncogene 2, anticorps v-akt murine thymoma viral oncogene homolog 2 L homeolog, anticorps AKT2, anticorps akt2, anticorps Akt2, anticorps akt2.L

Sujet: Name/Gene ID: AKT2
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB

ID gène: 208

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling