MAPK6 anticorps (AA 683-694)

N° du produit ABIN1911983

Détail du produit anti-MAPK6 anticorps

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Épitope: AA 683-694

Reactivité: Humain, Souris, Boeuf (Vache), Chien, Cheval, Porc, Lapin, Poulet, Hamster

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK6 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4, NP_081694.1).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-HSPVGSPLKSIQ from an internal region (near C-terminus) of human MAPK6 / ERK3 (NP_002739.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Rat, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:128000), WB (0.5 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 μg/mL. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MAPK6

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Autre désignation: MAPK6 / ERK3 (MAPK6 Produits)

Synonymes: anticorps ERK3, anticorps HsT17250, anticorps PRKM6, anticorps p97MAPK, anticorps 2610021I23Rik, anticorps D130053K17Rik, anticorps Erk3, anticorps Mapk4, anticorps Mapk63, anticorps Prkm4, anticorps Prkm6, anticorps ATMAPK6, anticorps ATMPK6, anticorps F18O19.10, anticorps MAP KINASE 6, anticorps MAP kinase 6, anticorps MAPK6, anticorps SUSERK3, anticorps erk3, anticorps p97mapk, anticorps si:ch211-235f12.1, anticorps mitogen-activated protein kinase 6, anticorps MAP kinase 6, anticorps MAPK6, anticorps Mapk6, anticorps MPK6, anticorps mapk6

Sujet: Name/Gene ID: MAPK6
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK

ID gène: 5597

NCBI Accession: NP_002739

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C