EIF2AK2 anticorps (pThr446)

N° du produit ABIN199580

Détail du produit anti-EIF2AK2 anticorps

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Épitope: pThr446

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2AK2 est non-conjugé

Application: Western Blotting (WB)

Specificité: Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.

Purification: Protein A purified

Immunogène: Peptide corresponding to amino acids surrounding Thr446 of human PKR.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-2.0 μg/mL detects phospho-PKR in RIPA lysates from HeLa cells that had. been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes. Beadlyte Peptide Specificity Assay: 1:1000-1:5000 dilutions were incubated with a cocktail of. microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKR (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur EIF2AK2

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Autre désignation: EIF2AK2 / PKR (EIF2AK2 Produits)

Synonymes: anticorps PKR, anticorps pkr, anticorps EIF2AK2, anticorps DKFZp469K1934, anticorps EIF2AK1, anticorps PRKR, anticorps 2310047A08Rik, anticorps 4732414G15Rik, anticorps AI467567, anticorps AI747578, anticorps Pkr, anticorps Prkr, anticorps Tik, anticorps eukaryotic translation initiation factor 2 alpha kinase 2, anticorps eukaryotic translation initiation factor 2-alpha kinase 2, anticorps EIF2AK2, anticorps eif2ak2, anticorps Eif2ak2

Sujet: Name/Gene ID: EIF2AK2
Subfamily: Translational Control Kinase
Family: Protein Kinase

Synonyms: EIF2AK2, p1/eIF-2A protein kinase, p68 kinase, PRKR, EIF-2A protein kinase 2, p68, Protein kinase RNA-activated, Tik

ID gène: 5610

UniProt: P19525

Pathways: Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C