MDM2 anticorps (AA 177-195)

N° du produit ABIN318856

Détail du produit anti-MDM2 anticorps

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Épitope: AA 177-195

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MDM2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation.

Purification: Purified

Immunogène: Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 3000 - 1: 12000. Western Blot: 1: 500 - 1: 2000, detects a band of approximately 90 kDa in mouse MEF celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MDM2

Antigène: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Autre désignation: MDM2 (MDM2 Produits)

Synonymes: anticorps ACTFS, anticorps HDMX, anticorps hdm2, anticorps zgc:109834, anticorps 1700007J15Rik, anticorps AA415488, anticorps Mdm-2, anticorps MDM2 proto-oncogene, anticorps MDM2 proto-oncogene S homeolog, anticorps Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse), anticorps MDM2 oncogene, E3 ubiquitin protein ligase, anticorps transformed mouse 3T3 cell double minute 2, anticorps MDM2, anticorps mdm2.S, anticorps Mdm2, anticorps mdm2

Sujet: Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination, however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

ID gène: 17246

NCBI Accession: NP_034916

UniProt: P23804

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway