MTOR anticorps (AA 2446-2450)
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- Antigène Voir toutes MTOR (mTOR) Anticorps
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Épitope
- AA 2446-2450
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp MTOR est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificité
- The antibody detects endogenous level of total mTOR protein.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
- Immunogène
- Peptide sequence around AA 2446-2450 (T-D-S-Y-S) derived from Human mTOR. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
- Isotype
- IgG
- Top Product
- Discover our top product mTOR Anticorps primaire
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- Indications d'application
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Antigène
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Autre désignation
- mTOR (mTOR Produits)
- Synonymes
- anticorps FRAP1, anticorps FRAP, anticorps FRAP2, anticorps RAFT1, anticorps RAPT1, anticorps 2610315D21Rik, anticorps AI327068, anticorps Frap1, anticorps flat, anticorps frap1, anticorps tor, anticorps wu:fc22h08, anticorps mechanistic target of rapamycin kinase, anticorps mechanistic target of rapamycin, anticorps mechanistic target of rapamycin (serine/threonine kinase), anticorps MTOR, anticorps Mtor, anticorps mtor
- Sujet
- Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
- Poids moléculaire
- 289 kDa
- NCBI Accession
- NP_004949
- UniProt
- P42345
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg
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