Insulin anticorps

N° du produit ABIN5540065

Détail du produit anti-Insulin anticorps

Antigène: Insulin (INS)

Reactivité: Porc

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp Insulin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID)

Specificité: The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography

Immunogène: Insulin hormone is isolated from Porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and Cytochemical Use: are usually between 1/100-1/250. In ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.

Restrictions: For Research Use only

Stockage

Buffer: PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. No foreign proteins added

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Insulin

Antigène: Insulin (INS)

Autre désignation: insulin (INS Produits)

Synonymes: anticorps IDDM2, anticorps ILPR, anticorps IRDN, anticorps MODY10, anticorps ins1, anticorps xins, anticorps ins1-a, anticorps Insulin, anticorps AA986540, anticorps Ins-2, anticorps InsII, anticorps Mody, anticorps Mody4, anticorps proinsulin, anticorps zgc:109842, anticorps igf2-A, anticorps ins, anticorps ins-a, anticorps ins-b, anticorps insulin, anticorps insulin precursor, anticorps insulin II, anticorps preproinsulin, anticorps insulin L homeolog, anticorps insulin S homeolog, anticorps INS, anticorps INS-IGF2, anticorps ins, anticorps Ins, anticorps PIN, anticorps Ins2, anticorps ins.L, anticorps ins.S

Sujet: Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.

UniProt: P01315

Pathways: Signalisation NF-kappaB, Signalisation RTK, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation