MAPK6 anticorps (C-Term, Internal Region)

N° du produit ABIN5541249

Détail du produit anti-MAPK6 anticorps

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Épitope: C-Term, Internal Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK6 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Séquence: C-HSPVGSPLKS IQ

Specificité: Recognizes MAPK6 / ERK3.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.

Information d'application

Indications d'application: Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N. Western blots of the IP were labelled with Rabbit anti-ERK3 serum (CST) or with Mouse anti-MK5 (SC). An approx 100 kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Tris saline, pH ~7.3 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur MAPK6

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Autre désignation: mapk6,erk3 (MAPK6 Produits)

Synonymes: anticorps ERK3, anticorps HsT17250, anticorps PRKM6, anticorps p97MAPK, anticorps 2610021I23Rik, anticorps D130053K17Rik, anticorps Erk3, anticorps Mapk4, anticorps Mapk63, anticorps Prkm4, anticorps Prkm6, anticorps ATMAPK6, anticorps ATMPK6, anticorps F18O19.10, anticorps MAP KINASE 6, anticorps MAP kinase 6, anticorps MAPK6, anticorps SUSERK3, anticorps erk3, anticorps p97mapk, anticorps si:ch211-235f12.1, anticorps mitogen-activated protein kinase 6, anticorps MAP kinase 6, anticorps MAPK6, anticorps Mapk6, anticorps MPK6, anticorps mapk6

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C