E-cadherin anticorps (CF®488A)
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- Antigène Voir toutes E-cadherin (CDH1) Anticorps
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp E-cadherin est conjugé à/à la CF®488A
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Fonction
- Mouse Monoclonal anti-E-Cadherin / CD324 (4A2), CF488A Conjugate
- Attributs du produit
- This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ?-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human full-length E-Cadherin protein
- Clone
- 4A2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDH1 Anticorps primaire
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- Indications d'application
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- Autre désignation
- E-Cadherin / CD324 (CDH1 Produits)
- Synonymes
- anticorps Arc-1, anticorps CD324, anticorps CDHE, anticorps ECAD, anticorps LCAM, anticorps UVO, anticorps E-cadherin, anticorps cdh1, anticorps si:dz180o5.2, anticorps uvo, anticorps lcam, anticorps E-Cad, anticorps XBcad, anticorps l-cam, anticorps xcdh1, anticorps cdhc-A, anticorps xb-cad, anticorps XTCAD-1, anticorps c-cadherin, anticorps cadherin-1, anticorps uvomorulin, anticorps XB-cadherin, anticorps arc-1, anticorps cd324, anticorps cdhe, anticorps ecad, anticorps AA960649, anticorps E-cad, anticorps Ecad, anticorps L-CAM, anticorps Um, anticorps cadherin 1, anticorps si:busm1-180o5.2, anticorps cadherin 1, type 1, E-cadherin (epithelial), anticorps cadherin 1, type 1 S homeolog, anticorps CDH1, anticorps si:busm1-180o5.2, anticorps cdh1, anticorps Cdh1, anticorps cdh1.S
- Sujet
- Arc 1|cadherin 1 type 1 E-cadherin|Cadherin1|CAM 120/80|CD324|CDH1|CDHE|E-Cad/CTF3|E-cadherin|ECAD|Epithelial cadherin|epithelial calcium dependent adhesion protein|Liver cell adhesion molecule (LCAM)|Uvomorulin (UVO)
- Poids moléculaire
- 120-80 kDa (Mature), 135 kDa (Precursor)
- ID gène
- 999, 461086
- UniProt
- P12830
- Pathways
- Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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