ERK2 Kit ELISA

N° du produit ABIN4993431

Détail du produit ERK2 Kit ELISA

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781-50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ERK2 in Rat serum, plasma and other biological fluids

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantRatERK2. No significant cross-reactivity or interference between RatERK2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatERK2 and all the analogues.

Sensibilité: 0.469 ng/mL

Ingrédients: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Matériel non inclus: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Information d'application

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ERK2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ERK2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ERK2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ERK2.You can calculate the concentration of ERK2in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Détail du antigène

Antigène: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Autre désignation: Extracellular Signal Regulated Kinase 2 (ERK2) (MAPK1 Produits)

Synonymes: ERK-1 Kit ELISA, ERK1 Kit ELISA, ERT2 Kit ELISA, HS44KDAP Kit ELISA, HUMKER1A Kit ELISA, P44ERK1 Kit ELISA, P44MAPK Kit ELISA, PRKM3 Kit ELISA, p44-ERK1 Kit ELISA, p44-MAPK Kit ELISA, Erk-1 Kit ELISA, Erk1 Kit ELISA, Ert2 Kit ELISA, Esrk1 Kit ELISA, Mnk1 Kit ELISA, Mtap2k Kit ELISA, Prkm3 Kit ELISA, p44 Kit ELISA, p44erk1 Kit ELISA, p44mapk Kit ELISA, ERK Kit ELISA, ERK2 Kit ELISA, ERT1 Kit ELISA, MAPK2 Kit ELISA, P42MAPK Kit ELISA, PRKM1 Kit ELISA, PRKM2 Kit ELISA, p38 Kit ELISA, p40 Kit ELISA, p41 Kit ELISA, p41mapk Kit ELISA, 9030612K14Rik Kit ELISA, AA407128 Kit ELISA, AU018647 Kit ELISA, C78273 Kit ELISA, Erk2 Kit ELISA, Prkm1 Kit ELISA, p42mapk Kit ELISA, zERK2 Kit ELISA, MAPK1 Kit ELISA, Xp42 Kit ELISA, erk Kit ELISA, erk2 Kit ELISA, ert1 Kit ELISA, mapk Kit ELISA, mapk1b Kit ELISA, mapk2 Kit ELISA, mpk1 Kit ELISA, prkm1 Kit ELISA, prkm2 Kit ELISA, mapk1 Kit ELISA, mapk1a Kit ELISA, xp42 Kit ELISA, ATMPK1 Kit ELISA, F14N23.9 Kit ELISA, F14N23_9 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, MNK1 Kit ELISA, ATMPK2 Kit ELISA, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Kit ELISA, T30E16.13 Kit ELISA, T30E16_13 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, mitogen-activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase 1 Kit ELISA, mitogen activated protein kinase 1 Kit ELISA, mitogen-activated protein kinase 1 L homeolog Kit ELISA, mitogen-activated protein kinase 1 S homeolog Kit ELISA, mitogen activated protein kinase 3 Kit ELISA, mitogen-activated protein kinase homolog 2 Kit ELISA, MAPK3 Kit ELISA, Mapk3 Kit ELISA, MAPK1 Kit ELISA, Mapk1 Kit ELISA, mapk1 Kit ELISA, mapk1.L Kit ELISA, mapk1.S Kit ELISA, PfMAP1 Kit ELISA, MPK1 Kit ELISA, PVX_084965 Kit ELISA, LOC9328617 Kit ELISA, Tsp_01601 Kit ELISA, MPK2 Kit ELISA

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins