TNF alpha Protéine

N° du produit ABIN1305134

Détail du produit

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Marque: BD Pharmingen™

Specificité: Biological Activity Range: Measured in a cytolysis assay using L929 indicator cells, Specific activity: 0.05 - 2.0 X 10^9 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.)
ED50: 5 - 200 pg/ml
Observed Dose response: 100 fold

Attributs du produit: Recombinant human TNF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered PBS, pH 7.2, and containing 4 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant human TNF was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant human TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives. Recombinant human TNF is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Pureté: ≥ 95 %

Stérilité: 0.22 μm filtered

niveau d'endotoxine: The endotoxin level is ≤ 0.10 ng/µg of human TNF, as measured in a chromogenic LAL assay.

Information d'application

Indications d'application: ELISA Standard: Recombinant human TNF is useful as a quantitative standard for measuring human TNF protein levels in a TNF specific sandwich ELISA with the purified MAb1 antibody (Cat. No. 551220) as a capture antibody and the biotinylated MAb11 antibody (Cat. No. 554511) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human TNF standard from 2000-15 pg/ml should be included in each ELISA plate. These ELISA reagents are not recommended for assay of serum samples. For testing TNF in serum or plasma, the human TNF BD OptEIA™ Set (Cat. No. 555212) and the human TNF BD OptEIA Kit (Cat. No. 550610) are specially formulated and recommended.

Commentaires:

BD Pharmingen™ Recombinant Human TNF - Reactivity Hu

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Agent conservateur: Azide free

Conseil sur la manipulation: Tumor Necrosis Factor (TNF, also known as TNF-α) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF is a 17.5 kD protein containing 157 amino acid residues.

Stock: -80 °C

Stockage commentaire: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Référence

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).

Wang, Creasey, Ladner, Lin, Strickler, Van Arsdell, Yamamoto, Mark: "Molecular cloning of the complementary DNA for human tumor necrosis factor." dans: Science (New York, N.Y.), Vol. 228, Issue 4696, pp. 149-54, (1985) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF (TNF alpha Produits)

Synonymes: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Sujet: Tumor Necrosis Factor (TNF, also known as TNF-α) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF is a 17.5 kD protein containing 157 amino acid residues.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome