TNF alpha Protéine

N° du produit ABIN2734614

Détail du produit

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human Tumor necrosis factor (TNF-alpha) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor (Tnf-Alpha) (TNF alpha Produits)

Synonymes: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Poids moléculaire: 17.3 kDa

NCBI Accession: NP_036807

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome