TNF alpha Protéine

N° du produit ABIN5775829

Détail du produit

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Attributs du produit: Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of actinomycin D is 1 x 10^7 units/mg.

Pureté: > 98 % pure

niveau d'endotoxine: < 0.1 ng per μg (1 EU/μg).

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of actinomycin D is 1 x 10^7 units/mg.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Buffer: Lyophilized from 5 mM tris, pH 8.0.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Référence

Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." dans: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF alpha (TNF alpha Produits)

Synonymes: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Sujet: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Alternative Names: TNF-a protein, Tumor Necrosis Factor protein, Necrosin protein, TNF-alpha protein, TNFa protein, TNFSF2 protein, DIF protein, Differentiation-inducing factor protein, Cachectin protein, Cytotoxin protein

Poids moléculaire: 17.3 kDa

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome