Phospholipase C gamma 1 anticorps (pTyr783, Tyr783) (Alexa Fluor 647)

N° du produit ABIN1177236

Détail du produit anti-Phospholipase C gamma 1 anticorps (Alexa Fluor 647)

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Épitope: pTyr783, Tyr783

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phospholipase C gamma 1 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated peptide including the tyrosine 783 of human PLCgamma1

Clone: 27-PLC

Isotype: IgG1

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-Phospholipase C gamma 1 (Alexa Fluor 647) (ABIN1177236)

Yu, Fukami, Itoh, Takenawa: "Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton." dans: Experimental cell research, Vol. 243, Issue 1, pp. 113-22, (1998) (PubMed).

Détails sur Phospholipase C gamma 1

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Autre désignation: PLC-gamma 1 (PLCG1 Produits)

Synonymes: anticorps NCKAP3, anticorps PLC-II, anticorps PLC1, anticorps PLC148, anticorps PLCgamma1, anticorps plc1, anticorps nckap3, anticorps plc-ii, anticorps plc148, anticorps plcgamma1, anticorps PPLCA, anticorps AI894140, anticorps Cded, anticorps Plc-1, anticorps Plc-gamma1, anticorps Plcg-1, anticorps fj46f04, anticorps wu:fj46f04, anticorps wu:fj57a05, anticorps phospholipase C gamma 1, anticorps phospholipase C gamma 1 L homeolog, anticorps 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, anticorps phospholipase C, gamma 1, anticorps PLCG1, anticorps plcg1.L, anticorps LOC100633163, anticorps Plcg1, anticorps plcg1

Sujet: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCg1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCg activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLCg1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612464) has been shown to react with human, mouse, and rat cell lysates by western blot.

Pathways: Signalisation RTK, Signalisation WNT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling