NF-kB p65 anticorps (pSer276)

N° du produit ABIN129575

Détail du produit anti-NF-kB p65 anticorps

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Épitope: pSer276

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S536 of human p65 (RelA) protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  By western blot, a band approximately 65 kDa in size corresponding to phosphorylated p65 (RelA) protein is expected in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human p65 (RelA) pS536 and shows minimal reactivity by western blot with non-phosphorylated p65 (RelA) and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-NF-kB p65 (ABIN129575)

Nakazawa, Oikawa, Ishii, Ayaki, Takahashi, Takeda, Ishitani, Kamei, Takeyoshi, Kawakami, Iwai, Hatada, Sawasaki, Ito, Nureki, Tokunaga: "Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis." dans: Nature communications, Vol. 7, pp. 12547, (2016) (PubMed).

Manna, Aggarwal, Sethi, Aggarwal, Ramesh et al.: "Morin (3,5,7,2',4'-Pentahydroxyflavone) abolishes nuclear factor-kappaB activation induced by various carcinogens and inflammatory stimuli, leading to suppression of nuclear factor-kappaB-regulated ..." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 7, pp. 2290-7, (2007) (PubMed).

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFkB p65 (NFkBP65 Produits)

Synonymes: anticorps C-Rel, anticorps NFKB3, anticorps p65, anticorps NFkB, anticorps c-rel, anticorps zgc:100833, anticorps Xrel2, anticorps Xrel3, anticorps rel, anticorps rel-A, anticorps rel2, anticorps rel3, anticorps v-rel, anticorps xrel, anticorps REL proto-oncogene, NF-kB subunit, anticorps RELA proto-oncogene, NF-kB subunit, anticorps v-rel reticuloendotheliosis viral oncogene homolog A (avian), anticorps v-rel avian reticuloendotheliosis viral oncogene homolog, anticorps v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog, anticorps REL, anticorps RELA, anticorps Rela, anticorps Rel, anticorps rel, anticorps rel.L

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB.  NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits.  Other identified subunits include p52 (NFKB2), cRel, and RelB.  The p65, cRel, and RelB subunits are responsible for transactivation.  The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers.  Lowlevels of p52 and p50 homodimers can also exist in cells.  The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a.  IkB-a binds to the p65 subunit, preventing nuclear localization, and DNA binding.  Activators mediate a rapid phosphorylation of IkB by IkB kinase (IKK), which results in subsequent ubiquitination and proteolytic degradation.  NFkB is then transported to the nucleus, where it activates transcription of target genes through binding to NFkB target sequences within the promoter.  The HTLV-I protein Tax can induce constitutive NFkB activation through phosphorylation of both IκB-α and IκB-β.  The transforming protein Tax inhibits p53 transcriptional activity through the NFkB signaling pathway, specifically via the p65 (RelA) subunit.  The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKβ.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

ID gène: 5970, 223468676

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins