c-MET anticorps (pTyr1249, pTyr1356)

N° du produit ABIN964593

Détail du produit anti-c-MET anticorps

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Épitope: pTyr1249, pTyr1356

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp c-MET est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using dual-phospho-peptide coupled to agarose beads followed by cross-adsorption against nonphospho-peptide.  This antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356.  A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence.  Cross-reactivity with c-Met from other sources has not been determined.

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K-Akt/PKB, Crk-Rap and Rac-Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 150 kDa in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Commentaires:

Gene Name: MET

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.01 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Détails sur c-MET

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: C-Met (MET Produits)

Synonymes: anticorps AUTS9, anticorps HGFR, anticorps RCCP2, anticorps c-Met, anticorps AI838057, anticorps HGF, anticorps Par4, anticorps Hgfr, anticorps c-met, anticorps MET, anticorps C-MET, anticorps met, anticorps met-A, anticorps MET proto-oncogene, receptor tyrosine kinase, anticorps met proto-oncogene, anticorps MET proto-oncogene, receptor tyrosine kinase L homeolog, anticorps MET, anticorps Met, anticorps met.L

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.
Synonyms: HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody

ID gène: 4233, 42741655

UniProt: P08581

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor