PKC beta anticorps (AA 601-673) (AbBy Fluor® 594)
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- Antigène Voir toutes PKC beta (PRKCB) Anticorps
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
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Épitope
- AA 601-673
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Reactivité
- Humain, Rat, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PKC beta est conjugé à/à la AbBy Fluor® 594
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Specificité
- This antibody will recognize isoform Beta-II & I of Protein kinase C beta
- Réactivité croisée
- Humain, Souris, Rat
- Homologie
- Cow,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic peptide derived from human PRKCB
- Isotype
- IgG
- Top Product
- Discover our top product PRKCB Anticorps primaire
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- Indications d'application
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))
- Autre désignation
- PKC beta (PRKCB Produits)
- Synonymes
- anticorps PKC-beta, anticorps PKCB, anticorps PRKCB1, anticorps PRKCB2, anticorps A130082F03Rik, anticorps PKC-Beta, anticorps Pkcb, anticorps Prkcb1, anticorps Prkcb2, anticorps PKC-B, anticorps Prkcb, anticorps prkcb1, anticorps zgc:63591, anticorps PKC, anticorps PRKCB_tv2, anticorps Pkc53E, anticorps prkcb1l, anticorps zgc:64063, anticorps protein kinase C beta, anticorps protein kinase C, beta, anticorps protein kinase C, beta b, anticorps protein kinase C, beta a, anticorps PRKCB, anticorps Prkcb, anticorps prkcbb, anticorps prkcba
- Sujet
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Synonyms: PKCB, PRKCB1, PRKCB2, PKC-beta, Protein kinase C beta type, PKC-B, PRKCB
Background: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4.
- ID gène
- 5579
- UniProt
- P05771
- Pathways
- Signalisation WNT, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling
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