Recombinant ADAM17 anticorps (His tag)

N° du produit ABIN5564274

Détail du produit anti-ADAM17 anticorps (His tag)

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp ADAM17 est conjugé à/à la His tag

Application: Functional Studies (Func)

Specificité: Recognizes the catalytic and non-catalytic domain of human ADAM17 (TACE) through its variable light (VL) domain and variable heavy (VH) domain, respectively. Does not bind recombinant mouse ADAM17 ectodomain.

Réactivité croisée: Humain

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.001EU/μg

Immunogène: Recombinant human ADAM17 (TACE) ectodomain tagged to biotin.

Clone: D1(A12)

Isotype: Kappa

Information d'application

Commentaires:

This cross-domain human antibody is a selective ADAM17 (TACE) antagonist and provides a unique alternative to small molecule metalloprotease inhibition. ADAM multidomain topology was exploited by first isolating an inhibitory human antibody (D1) that bound ADAM17-specific non-catalytic regions exclusively through its variable heavy (VH) domain. A D1-VH biased scFv phage-display library was then used to selectively isolate a new variable light (VL) chain that could simultaneously bind to the ADAM17 catalytic domain. This monovalent Fab Antibody has been shown by C.J. Tape, et al., PNAS 108, 5578 (2011) to be as active as the divalent antibody .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C.

Date de péremption: 12 months

Détails sur ADAM17

Antigène: ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))

Autre désignation: ADAM17 (ADAM17 Produits)

Synonymes: anticorps ADAM18, anticorps CD156B, anticorps CSVP, anticorps NISBD, anticorps TACE, anticorps CD156b, anticorps Tace, anticorps adam17, anticorps si:dkey-81b15.2, anticorps adam18, anticorps cd156b, anticorps csvp, anticorps tace, anticorps fc20b01, anticorps wu:fc20b01, anticorps zgc:63886, anticorps ADAM metallopeptidase domain 17, anticorps a disintegrin and metallopeptidase domain 17, anticorps ADAM metallopeptidase domain 17b, anticorps ADAM metallopeptidase domain 17 L homeolog, anticorps ADAM metallopeptidase domain 17a, anticorps ADAM17, anticorps Adam17, anticorps adam17b, anticorps adam17.L, anticorps adam17a

Sujet: ADAM17 (Disintegrin and metalloproteinase domain-containing protein 17), also called TACE (Tumor necrosis factor-alpha-converting enzyme) is the prototype of the ADAM family of ectodomain shedding proteases (sheddase). ADAM17 is understood to be involved in the processing of TNF-alpha at the surface of the cell and from within the intracellular membranes of the trans-Golgi network. This process involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha) and is of known physiological importance. ADAM17 is responsible for the processing of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands and enzymes, including cleaving epidermal growth factor receptor (EGFR) ligands and extracellular Notch1. The proteolytic cleavage is an indispensable activation event for many of these substrates, ADAM17 has emerged as an attractive therapeutic target for the treatment of inflammatory diseases (e.g. rheumatoid arthritis) or inflammation associated cancer.

UniProt: P78536

Pathways: Signalisation Notch, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus