PTPN11 Kit ELISA

N° du produit ABIN2747888

Détail du produit PTPN11 Kit ELISA

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Épitope: pTyr542

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Cell-Based Human/Mouse SHP2 (Y542) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.

Type d'échantillon: Cell Culture Cells

Analytical Method: Semi-Quantitative

Specificité: The antibodies provided in this kit recognizes human and mouse SHP2 phosphorylated at Tyrosine-542 and total SHP2 for comparison.

Attributs du produit:

• Site and signal pathway-specific
• In vitro detection of adherent cell culture
• No sample lysis needed
• Compatible with a standard ELISA plate reader
• Faster results than with ELISA
• Adaptable for high-throughput screening and drug discovery

Ingrédients:

• uncoated 96-well Microplate
• Wash Buffer A
• Wash Buffer B
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Uncoated

Protocole:

1. Seed 10,000-30,000 cells into each well and incubate overnight.
2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Autre désignation: SHP2 (PTPN11 Produits)

Synonymes: BPTP3 Kit ELISA, CFC Kit ELISA, NS1 Kit ELISA, PTP-1D Kit ELISA, PTP2C Kit ELISA, SH-PTP2 Kit ELISA, SH-PTP3 Kit ELISA, SHP2 Kit ELISA, 2700084A17Rik Kit ELISA, AW536184 Kit ELISA, PTP1D Kit ELISA, SAP-2 Kit ELISA, SHP-2 Kit ELISA, Shp2 Kit ELISA, Syp Kit ELISA, SYP Kit ELISA, bptp3 Kit ELISA, cfc Kit ELISA, ns1 Kit ELISA, ptp-2 Kit ELISA, ptp2c Kit ELISA, ptpn11 Kit ELISA, ptpn11-a Kit ELISA, ptpn11-b Kit ELISA, shp-2 Kit ELISA, shp2 Kit ELISA, fa14b09 Kit ELISA, wu:fa14b09 Kit ELISA, wu:fi24f03 Kit ELISA, zgc:55388 Kit ELISA, zgc:63553 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 S homeolog Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, a Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, b Kit ELISA, PTPN11 Kit ELISA, Ptpn11 Kit ELISA, ptpn11.S Kit ELISA, ptpn11a Kit ELISA, ptpn11b Kit ELISA

Classe de substances: Viral Protein

Sujet: Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D (PTP-1D), Protein-tyrosine phosphatase 2C (PTP-2C), SH-PTP2 (SHP-2), SH-PTP3

ID gène: 5781

UniProt: Q06124

Pathways: Signalistation JAK/STAT, Signalisation RTK, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, L'effet Warburg