IKBKB Kit ELISA

N° du produit ABIN6951455

Détail du produit IKBKB Kit ELISA

Antigène: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human IKK-B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Autre désignation: IKK-B (IKBKB Produits)

Synonymes: ikk-beta Kit ELISA, ikk2 Kit ELISA, ikkb Kit ELISA, ikkbeta Kit ELISA, IKBKB Kit ELISA, IKKB Kit ELISA, CG4201 Kit ELISA, DIK Kit ELISA, DLAK Kit ELISA, DmIKK Kit ELISA, DmIKKBeta Kit ELISA, DmIKKb Kit ELISA, DmIKKbeta Kit ELISA, DmIkkbeta Kit ELISA, Dmel\\CG4201 Kit ELISA, Dmikkb Kit ELISA, Dmikkbeta Kit ELISA, IK Kit ELISA, IKK Kit ELISA, IKK-beta Kit ELISA, IKKb Kit ELISA, IKKbeta Kit ELISA, IRD-5 Kit ELISA, IRD5 Kit ELISA, Ik Kit ELISA, Ikkb Kit ELISA, Ikkbeta Kit ELISA, Ird-5 Kit ELISA, Ird5 Kit ELISA, LRK Kit ELISA, Lak Kit ELISA, anon-89Bd Kit ELISA, dIKK Kit ELISA, dIKK-beta Kit ELISA, dIKKbeta Kit ELISA, dLak Kit ELISA, dmIKKbeta Kit ELISA, ikk Kit ELISA, ird Kit ELISA, ird5/DmIkk[beta] Kit ELISA, IKK2 Kit ELISA, NFKBIKB Kit ELISA, AIM-1 Kit ELISA, AI132552 Kit ELISA, IKK-2 Kit ELISA, IKK[b] Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit beta Kit ELISA, I-kappaB kinase beta Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta Kit ELISA, inhibitor of kappaB kinase beta Kit ELISA, ikbkb.L Kit ELISA, IKBKB Kit ELISA, ikbkb Kit ELISA, IKKbeta Kit ELISA, Ikbkb Kit ELISA

ID gène: 3551

UniProt: O14920

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins