PTPN11 anticorps (pTyr542) (PE)

N° du produit ABIN1177171

Détail du produit anti-PTPN11 anticorps (PE)

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Épitope: pTyr542

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PTPN11 est conjugé à/à la PE

Application: Western Blotting (WB), Intracellular Staining (ICS)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human SHP2 Peptide

Clone: L99-921

Isotype: IgG1 kappa

Information d'application

Indications d'application: This antibody conjugate is suitable for intracellular staining of cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Références pour anticorps anti-PTPN11 (PE) (ABIN1177171)

Fornasa, Groyer, Clement, Dimitrov, Compain, Gaston, Varthaman, Khallou-Laschet, Newman, Graff-Dubois, Nicoletti, Caligiuri: "TCR stimulation drives cleavage and shedding of the ITIM receptor CD31." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 10, pp. 5485-92, (2010) (PubMed).

MacGillivray, Herrera-Abreu, Chow, Shek, Wang, Vachon, Feng, Siminovitch, McCulloch, Downey: "The protein tyrosine phosphatase SHP-2 regulates interleukin-1-induced ERK activation in fibroblasts." dans: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27190-8, (2003) (PubMed).

Lu, Gong, Bar-Sagi, Cole: "Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling." dans: Molecular cell, Vol. 8, Issue 4, pp. 759-69, (2001) (PubMed).

Bennett, Tang, Sugimoto, Walsh, Neel: "Protein-tyrosine-phosphatase SHPTP2 couples platelet-derived growth factor receptor beta to Ras." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 7335-9, (1994) (PubMed).

Ahmad, Banville, Zhao, Fischer, Shen: "A widely expressed human protein-tyrosine phosphatase containing src homology 2 domains." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 6, pp. 2197-201, (1993) (PubMed).

Détails sur PTPN11

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Autre désignation: SHP2 (PTPN11 Produits)

Synonymes: anticorps BPTP3, anticorps CFC, anticorps NS1, anticorps PTP-1D, anticorps PTP2C, anticorps SH-PTP2, anticorps SH-PTP3, anticorps SHP2, anticorps 2700084A17Rik, anticorps AW536184, anticorps PTP1D, anticorps SAP-2, anticorps SHP-2, anticorps Shp2, anticorps Syp, anticorps SYP, anticorps bptp3, anticorps cfc, anticorps ns1, anticorps ptp-2, anticorps ptp2c, anticorps ptpn11, anticorps ptpn11-a, anticorps ptpn11-b, anticorps shp-2, anticorps shp2, anticorps fa14b09, anticorps wu:fa14b09, anticorps wu:fi24f03, anticorps zgc:55388, anticorps zgc:63553, anticorps protein tyrosine phosphatase, non-receptor type 11, anticorps protein tyrosine phosphatase, non-receptor type 11 S homeolog, anticorps protein tyrosine phosphatase, non-receptor type 11, a, anticorps protein tyrosine phosphatase, non-receptor type 11, b, anticorps PTPN11, anticorps Ptpn11, anticorps ptpn11.S, anticorps ptpn11a, anticorps ptpn11b

Classe de substances: Viral Protein

Sujet: SHP2 is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 reportedly contains two SH2 domains, both of which are N-terminal to the PTP catalytic domain. SH2 PTPs are believed to work in conjunction with protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. SHP2 is phosphorylated at two C-terminal tyrosine residues: Y542 and Y580. Phosphorylation of Y542 creates an interaction in the N-terminal SH2 domain and appears to relieve basal inhibition of the PTP, while phosphorylation at Y580 creates an interaction at the C-terminal that stimulates PTP activity. The L99-921 monoclonal antibody recognizes the phosphorylated Y542 of SHP2 (PTP2C isoform 2). The homologous phosphorylation site in the PTP2Ci splice variant (isoform 1) is Y546. The specificity of this antibody was validated by confirming, using western blot analysis, that RNA-mediated interference (RNAi) of the specific protein was able to down-regulate the expression of SHP2 (pY542).
Synonyms: PTN11, PTP1D, PTP2C, PTPN11, SH-PTP2, SH-PTP3, SHP-2, SHPTP2

Pathways: Signalistation JAK/STAT, Signalisation RTK, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, L'effet Warburg