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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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254 Résultats

MEK1 anticorps (AA 2-150)

MAP2K1 Reactivité: Humain ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

IHC

Image provided by the Independent Validation Program (badge number 29760).

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (ABIN686482) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

IHC

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

IHC (p)

7 images

1 validation

N° du produit ABIN686482

Détails

Independently Validated

PI3K p85 alpha/gamma anticorps (pTyr199, pTyr467)

PI3K p85a/g Reactivité: Humain, Souris, Rat, Lapin WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

5 images

3 références

1 validation

N° du produit ABIN744743

Détails

Independently Validated

Histone H4 anticorps (acLys12)

Reactivité: Humain, Souris, Rat WB, IHC, IF, ELISA, ICC Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Histone H4 (acLys12) antibody (ABIN6254899)

IHC

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Acetyl-Histone H4 (Lys12) Antibody.

IF/ICC

Western blot analysis of extracts from mouse spleen, using Acetyl-Histone H4 (Lys12) Antibody.

ABIN6267063 at 1/100 staining human Brain tissue sections by IHC-P.

IHC

Western blot analysis of extracts from various samples, Acetyl-Histone H4 (Lys12) Antibody.

6 images

1 validation

N° du produit ABIN6254899

Détails

Independently Validated

GLI3 anticorps

GLI3 Reactivité: Humain WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

CUT&RUN

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

IHC

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

IHC

Expression of genes and proteins related to Hh signaling in SLC mice.

IHC

10 images

1 validation

N° du produit ABIN2855813

Détails

Independently Validated

Cortactin anticorps (N-Term)

KO Validated CTTN Reactivité: Humain WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Cortactin (CTTN) (N-Term) antibody (ABIN2854674)

mIHC

WB Image Sample (30 ug of whole cell lysate) A: PC-12 7.

WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin, antibody at 1:250 dilution.

IHC

ICC/IF Image Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.

7 images

1 validation

N° du produit ABIN2854674

Détails

Independently Validated

NFKB1 anticorps (AA 860-969)

NFKB1 Reactivité: Humain WB, ELISA, IF, IHC (p), PLA Hôte: Souris Monoclonal 4A11 unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody (ABIN561981)

Western Blot detection against Immunogen (37.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon.

ISt

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

ELISA

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1.

PLA

8 images

1 validation

N° du produit ABIN561981

Détails

Independently Validated

SYN1 anticorps (AA 362-511)

SYN1 Reactivité: Humain, Rat, Souris, Singe WB, ELISA, IHC, ICC, FACS Hôte: Souris Monoclonal 7H10G6 unconjugated

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

mIHC

Western blot analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein.

Immunohistochemical analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.

IHC

Western blot analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.

Flow cytometric analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).

12 images

1 validation

N° du produit ABIN5542390

Détails

Independently Validated

TLR9 anticorps (AA 868-1016)

TLR9 Reactivité: Humain IHC, ELISA, FACS, ICC Hôte: Souris Monoclonal 1B12H2 unconjugated

Immunofluorescence validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Proximity Ligation Assay validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

9 images

2 validations

N° du produit ABIN5542492

Détails

Independently Validated

CREB1 anticorps

CREB1 Reactivité: Humain, Souris, Rat, Singe WB, IHC, ELISA, ICC Hôte: Souris Monoclonal 5G3 unconjugated

Western Blotting validation image for anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061)

Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.

IHC

Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng),

ELISA

Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green).

5 images

3 références

1 validation

N° du produit ABIN969061

Détails

Independently Validated

NeuN anticorps (AA 1-140)

RBFOX3 Reactivité: Humain, Souris WB, ELISA, FACS, ICC Hôte: Souris Monoclonal 1H3B2 unconjugated

Western Blotting validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Immunohistochemistry validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

IHC

Western blot analysis using RBFOX3 mAb against human RBFOX3 (AA: 1-140) recombinant protein.

Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green).

ICC

ELISA

Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.

Flow cytometric analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).

FACS

8 images

2 validations

N° du produit ABIN5542704

Détails

Independently Validated

APP anticorps (C-Term)

APP Reactivité: Humain WB, IF, ICC, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody (ABIN2855011)

IHC

WB Image Sample(30 μg of whole cell lysate) A:Hep G2, 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP, antibody(10 μg/ml).

IHC

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm and nucleus by immunofluorescent analysis.

IHC-P Image APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.

IHC

WB Image Various whole cell extracts (30 μg) were separated by 7.

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.

ICC/IF Image APP antibody [C2C3], C-term detects APP protein at cytoplasm by confocal immunofluorescent analysis.

8 images

1 validation

N° du produit ABIN2855011

Détails

Independently Validated

ARPC2 anticorps

ARPC2 Reactivité: Humain WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

Multiplex Immunohistochemistry validation image for anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody (ABIN2855249)

mIHC

WB Image ARPC2 antibody detects ARPC2 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2, antibody at 1:100 dilution.

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2, antibody at 1:200 dilution.

WB Image Sample(30 μg of whole cell lysate) A:293T B: HeLa S3, 12% SDS PAGE antibody diluted at 1:500

7 images

1 validation

N° du produit ABIN2855249

Détails

Independently Validated

Histone 3 anticorps (3meLys4)

H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone H3 (H3) (3meLys4) antibody (ABIN6971977)

CUT&RUN

Histone H3K4me3 antibody tested by Western blot.

Histone H3 trimethyl Lys4 antibody tested by dot blot analysis.

Detection of H3K4me3 by immunofluorescence U2OS cells were stained with H3K4me3 antibody at a dilution of 1:500.

ChIP-seq

6 images

1 validation

N° du produit ABIN6971977

Détails

Independently Validated

Clathrin anticorps (AA 4-171)

Reactivité: Humain, Souris, Rat, Poulet, Chien IHC, WB, IF, IP, BI Hôte: Souris Monoclonal 23-Clathrin Heavy Chain unconjugated

Immunohistochemistry validation image for anti-Clathrin (AA 4-171) antibody (ABIN968006)

IHC

Rat cerebellum, zinc-fixed paraffin embedded tissue (left), 40X

IHC (p)

Western blot analysis of Clathrin Heavy Chain on HeLa cell lysate (center).

Immunofluorescent staining of C6 cells (right).

7 images

5 références

1 validation

N° du produit ABIN968006

Détails

Independently Validated

beta Actin anticorps (AA 2-16)

ACTB Reactivité: Souris, Rat, Poulet, Poisson zèbre (Danio rerio) WB, IHC, ICC Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

ICC

Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

IHC

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle section (dilution 1 : 500; red).

IHC (p)

7 images

3 références

1 validation

N° du produit ABIN1742508

Détails

Independently Validated

APP anticorps

Rockland 600-401-253 APP Reactivité: Humain, Souris WB, IHC, ELISA, IF, FM Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

IHC

Western Blot of Rabbit anti-Beta Amyloid antibody.

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

IHC

ELISA

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC

Immunofluorescence (IF) image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037)

9 images

1 reference

2 validations

N° du produit ABIN95037

Détails

Independently Validated

COL3A1 anticorps

Rockland 600-401-105-0.1 COL3A1 Reactivité: Humain, Boeuf (Vache), Porc WB, IHC, ELISA, IP, FACS, DB, FM Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

IHC

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

IHC

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

IHC

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 références

1 validation

N° du produit ABIN5596830

Détails

Independently Validated

Collagen IV anticorps

Rockland 600-401-106-0.1 COL4 Reactivité: Humain, Boeuf (Vache) WB, ELISA, IHC, IP, DB, FM Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

IHC

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

12 images

8 références

1 validation

N° du produit ABIN5596835

Détails

Independently Validated

Histone 3 anticorps (H3K9ac)

H3 Reactivité: Humain, Souris WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone H3 (H3) (H3K9ac) antibody (ABIN2667854)

CUT&RUN

Western blot of Histone H3 acetyl Lys9 antibody (pAb).

ChIP

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.

ChIP-seq

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.

7 images

3 références

1 validation

N° du produit ABIN2668415

Détails

Independently Validated

Histone 3 anticorps (H3K36me3)

H3 Reactivité: Humain, Souris WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Souris Monoclonal MABI 0333 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone H3 (H3) (H3K36me3) antibody (ABIN2668403)

CUT&RUN

Western blot of Histone H3 trimethyl Lys36 antibody.

Dot blot of Histone H3 trimethyl Lys36 antibody.

ChIP-seq

Histone H3 dimethyl Lys36 antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Lys36 antibody tested by Western blot.

7 images

4 références

1 validation

N° du produit ABIN2668403

Détails

Independently Validated

SNAP Tag anticorps

ELISA, SEM, WB Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-SNAP Tag antibody (ABIN1573927)

Immunofluorescence validation image for anti-SNAP Tag antibody (ABIN1573927)

Electron Microscopy validation image for anti-SNAP Tag antibody (ABIN1573927)

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD) Lane 3: Negative 293 cell lysate Primary antibody: 1 µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (ABIN398494) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 10,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939)

4 images

3 références

3 validations

N° du produit ABIN1573927

Détails

Independently Validated

CD84 anticorps (Internal Region)

CD84 Reactivité: Humain, Souris WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD84 (CD84) (Internal Region) antibody (ABIN3187786)

IHC

Western Blotting (WB) image for anti-CD84 (CD84) (Internal Region) antibody (ABIN3187786)

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

IHC

5 images

1 validation

N° du produit ABIN3187786

Détails

Independently Validated

RUNX3 anticorps (AA 31-130)

RUNX3 Reactivité: Humain WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Runx3 Polyclonal Antibody, Unconjugated (ABIN1714069) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

5 images

1 validation

N° du produit ABIN1714069

Détails

Independently Validated

MYCN anticorps (AA 401-464)

MYCN Reactivité: Humain, Souris ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-N-Myc Proto-Oncogene Protein (MYCN) (AA 401-464) antibody (ABIN760676)

Image provided by the Independent Validation Program (badge number 29800).

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human brain labeled with Anti-n-Myc Polyclonal Antibody, Unconjugated (ABIN760676) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

4 images

1 reference

1 validation

N° du produit ABIN760676

Détails

Independently Validated

TTF1 anticorps (AA 201-300)

TTF1 Reactivité: Humain, Rat, Souris WB, FACS, ICC, IF (cc) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

SDS

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

IF (cc)

4 images

2 références

1 validation

N° du produit ABIN728713

Détails

Independently Validated

IGF1R anticorps (AA 251-350)

IGF1R Reactivité: Humain, Souris, Rat, Chien FACS Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody (ABIN726575)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .

FACS

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

2 références

1 validation

N° du produit ABIN726575

Détails

Independently Validated

CDH6 anticorps (AA 281-380)

CDH6 Reactivité: Humain WB, ELISA, FACS Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

5 images

1 validation

N° du produit ABIN715286

Détails

Independently Validated

FZD7 anticorps (AA 501-574)

FZD7 Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 501-574) antibody (ABIN710051)

Image provided by the Independent Validation Program (badge number 29805).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

4 images

1 reference

1 validation

N° du produit ABIN710051

Détails

Independently Validated

MTOR anticorps (AA 2436-2492)

mTOR Reactivité: Humain, Souris, Rat WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody (ABIN676403)

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated and DAPI staining

IHC

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

9 références

1 validation

N° du produit ABIN676403

Détails

Independently Validated

Cadherin anticorps (AA 841-882)

Reactivité: Humain, Souris WB, FACS, ICC, IF (cc), IF (p), IHC (p), ELISA, IHC (fro) Hôte: Lapin Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

FACS

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

4 images

2 références

1 validation

N° du produit ABIN687682

Détails

Independently Validated

LRP2 anticorps (AA 3401-3500) (Cy3)

LRP2 Reactivité: Humain, Souris FACS, IF (cc), IF (p) Hôte: Lapin Polyclonal Cy3

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

5 images

1 validation

N° du produit ABIN750991

Détails

Independently Validated

TCF7L2 anticorps

TCF7L2 Reactivité: Humain, Souris, Rat WB, IHC, IF (cc), IHC (p), FACS Hôte: Lapin Monoclonal 2C2 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) antibody (ABIN6945139)

CUT&RUN

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

CUT&RUN

4 images

1 reference

1 validation

N° du produit ABIN6945139

Détails

Independently Validated

STAT5A anticorps

STAT5A Reactivité: Humain, Souris, Rat WB, IHC (p), IF (cc) Hôte: Lapin Monoclonal 6E5 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 5A (STAT5A) antibody (ABIN5557532)

CUT&RUN

Lane 1: Hela, Lane 2: K562 cell lysates, probed with STAT5a (6E5) Monoclonal Antibody at 1:1000 overnight at 4˚C.

Paraformaldehyde-fixed, paraffin embedded human tonsil section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with STAT5a (6E5) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

HeLa cells were stained with STAT5a (6E5) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

IF (cc)

5 images

1 validation

N° du produit ABIN5557532

Détails

Independently Validated

TBX2/3 anticorps

Reactivité: Humain, Souris, Rat WB, ICC, IF Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-TBX2/3 antibody (ABIN6265491)

CUT&RUN

Western blot analysis of TBX3 expression in mouse brain lysate

IF/ICC

Western blot analysis on A549 cell lysate using TBX3 Antibody.

5 images

1 validation

N° du produit ABIN6265491

Détails

Independently Validated

ELF1 anticorps (C-Term)

Verified ELF1 Reactivité: Humain, Souris WB, ELISA, IHC Hôte: Chèvre Polyclonal unconjugated

Western Blotting validation image for anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody (ABIN184790)

IHC

5 images

1 validation

N° du produit ABIN184790

Détails

Independently Validated

Histone H4 anticorps (3meLys20)

Reactivité: Humain, Souris, Rat WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Histone H4 (3meLys20) antibody (ABIN7260401)

IHC

Western blot analysis of extracts of various cell lines using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:1000.

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Polyclonal Antibody

Immunohistochemistry of paraffin-embedded Rat testis using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:200 (40x lens).

IHC (p)

Immunohistochemistry of paraffin-embedded Mouse testis using TriMethyl-Histone H4-K20 Polyclonal Antibody at dilution of 1:200 (40x lens).

IHC (p)

5 images

1 validation

N° du produit ABIN7260402

Détails

Independently Validated

DLG4 anticorps

DLG4 Reactivité: Rat WB, IHC, ICC, IF, AA, mIHC Hôte: Souris Monoclonal 6G6 unconjugated

Multiplex Immunohistochemistry validation image for anti-Discs, Large Homolog 4 (Drosophila) (DLG4) antibody (ABIN361694)

mIHC

western rat membrane 1 in 1000 PSD 95 copy.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PSD95 Monoclonal Antibody, Clone 6G6 (ABIN361693 and ABIN361694).

ICC

5 images

1 validation

N° du produit ABIN361694

Détails

Independently Validated

SLC17A7 anticorps (AA 493-560)

SLC17A7 Reactivité: Rat WB, IHC, mIHC Hôte: Souris Monoclonal S28-9 unconjugated

Multiplex Immunohistochemistry validation image for anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (ABIN1027710)

mIHC

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (ABIN1027710).

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (ABIN1027710).

ICC

5 images

1 validation

N° du produit ABIN1027710

Détails

Independently Validated

SMAD4 anticorps (N-Term)

SMAD4 Reactivité: Humain WB, IP, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody (ABIN6972727)

CUT&RUN

SMAD4 antibody (pAb) tested by Western blot.

SMAD4 antibody (pAb) tested by Immunoprecipitation.

4 images

1 reference

1 validation

N° du produit ABIN6972727

Détails

Independently Validated

MR1 anticorps (AA 201-300)

MR1 Reactivité: Humain ELISA, WB, IF Hôte: Souris Monoclonal 5B5 unconjugated

Immunocytochemistry validation image for anti-Major Histocompatibility Complex, Class I-Related (MR1) (AA 201-300) antibody (ABIN516526)

ICC

Immunoprecipitation validation image for anti-Major Histocompatibility Complex, Class I-Related (MR1) (AA 201-300) antibody (ABIN516526)

Flow Cytometry validation image for anti-Major Histocompatibility Complex, Class I-Related (MR1) (AA 201-300) antibody (ABIN516526)

FACS

Western Blot detection against Immunogen (36.

Immunofluorescence of monoclonal antibody to MR1 on HeLa cell.

5 images

3 validations

N° du produit ABIN516526

Détails

Independently Validated

TRPC5 anticorps (AA 827-845) (FITC)

TRPC5 Reactivité: Humain WB, IHC, IP, AA Hôte: Souris Monoclonal N67-15 (Formerly S67-15) FITC

Immunofluorescence validation image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5) (AA 827-845) antibody (FITC) (ABIN2485379)

Western Blot analysis of Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15 .

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (ABIN2485379).

ICC

Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15 .

IHC

Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (ABIN2485379).

5 images

1 validation

N° du produit ABIN2485379

Détails

Independently Validated

S1PR1 anticorps (C-Term)

S1PR1 Reactivité: Humain WB, IF, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Sphingosine-1-Phosphate Receptor 1 (S1PR1) (C-Term) antibody (ABIN2857018)

WB Image EDG1 antibody [C2C3], C-term detects S1PR1 protein by Western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1, antibody at 1:500 dilution.

IHC

WB Image Sample(30 μg of whole cell lysate) A:Hep G2 , 12% SDS PAGE antibody diluted at 1:1000

ICC/IF Image EDG1 antibody [C2C3], C-term detects EDG1 protein at cytoplasm by immunofluorescent analysis.

5 images

1 validation

N° du produit ABIN2857018

Détails

Independently Validated

TCF7 anticorps (N-Term)

TCF7 Reactivité: Humain WB, IHC, IP, CUT&RUN Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7 (T-Cell Specific, HMG-Box) (TCF7) (N-Term) antibody (ABIN5620945)

CUT&RUN

CUT&RUN

4 images

1 reference

1 validation

N° du produit ABIN5620945

Détails

Independently Validated

Androgen Receptor anticorps

AR Reactivité: Humain, Souris, Rat WB, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Androgen Receptor (AR) antibody (ABIN1871059)

Western Blotting (WB) image for anti-Androgen Receptor (AR) antibody (ABIN1871059)

Western blot analysis of extracts of 22RV1 and mouse heart cell lines, using AR antibody.

Immunohistochemistry of paraffin-embedded mouse brain using AR antibody.

IHC

Immunofluorescence analysis of A549 cell using AR antibody.

Western blot analysis of extracts of 22RV1 cells, using AR Antibody (ABIN1871059) at 1:500 dilution.

6 images

1 validation

N° du produit ABIN1871059

Détails

Independently Validated

TUBB3 anticorps (AA 443-450)

TUBB3 Reactivité: Humain, Souris, Rat WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody (ABIN1742553)

Western Blotting (WB) image for anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody (ABIN1742553)

Immunocytochemistry (ICC) image for anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody (ABIN1742553)

ICC

Immunohistochemistry (IHC) image for anti-Tubulin, beta 3 (TUBB3) (AA 443-450) antibody (ABIN1742553)

IHC

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse brain section (dilution 1 : 500; red).

IHC (p)

5 images

1 reference

1 validation

N° du produit ABIN1742553

Détails

Independently Validated

CYP4B1 anticorps (N-Term)

CYP4B1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Chévre WB Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Cytochrome P450, Family 4, Subfamily B, Polypeptide 1 (CYP4B1) (N-Term) antibody (ABIN2777006)

Western Blotting validation image for anti-Cytochrome P450, Family 4, Subfamily B, Polypeptide 1 (CYP4B1) (N-Term) antibody (ABIN2777006)

WB Suggested Anti-CYP4B1 Antibody Titration: 0.

Host: Rabbit Target Name: CYP4B1 Sample Type: Human Fetal Lung Antibody Dilution: 1.

Host: Rabbit Target Name: CYP4B1 Sample Type: Human Fetal Liver Antibody Dilution: 1.

6 images

2 validations

N° du produit ABIN2777006

Détails

Independently Validated

HYAL1 anticorps (N-Term)

HYAL1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin WB, IHC Hôte: Lapin Polyclonal unconjugated

Western Blotting validation image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784342)

Host: Rabbit Target Name: HYAL1 Sample Type: Human Fetal Lung Antibody Dilution: 1.

Immunohistochemistry (IHC) image for anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody (ABIN2784342)

IHC

Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.

IHC

HYAL1 antibody - N-terminal region validated by WB using HepG2 cell lysate at 1ug/ml.

Host: Rabbit Target Name: HYAL1 Sample Type: Human Fetal Brain Antibody Dilution: 1.

6 images

1 validation

N° du produit ABIN2784342

Détails

Independently Validated

APP anticorps (AA 666-670)

APP Reactivité: Humain, Souris, Rat WB, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433)

Image no. 2 for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197432)

Image no. 1 for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197432)

6 images

1 validation

N° du produit ABIN197432

Détails

Independently Validated

MAP2 anticorps

MAP2 Reactivité: Humain, Souris, Porc WB, ELISA, ICC, IP, IHC (p), IHC (fro) Hôte: Souris Monoclonal MT-08 unconjugated

Multiplex Immunohistochemistry validation image for anti-Microtubule-Associated Protein 2 (MAP2) antibody (ABIN125739)

mIHC

Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate.

3 images

1 reference

1 validation

N° du produit ABIN125739

Détails

Independently Validated

RDH12 anticorps (AA 1-316)

RDH12 Reactivité: Humain ELISA, IHC Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Retinol Dehydrogenase 12 (All-Trans/9-Cis/11-Cis) (RDH12) (AA 1-316) antibody (ABIN7167836)

IHC

Immunohistochemistry of paraffin-embedded human gastric cancer using ABIN7167836 at dilution of 1:100

IHC

Immunohistochemistry of paraffin-embedded human kidney tissue using ABIN7167836 at dilution of 1:100

IHC

3 images

1 reference

1 validation

N° du produit ABIN7167836

Détails

Independently Validated

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