AKT1 Protein (His tag)

N° du produit ABIN7491341

Détail du produit

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette AKT1 protéine est marqué à la His tag.

Fonction: Recombinant Human AKT1 Protein with C-terminal 8xHis tag

Specificité: AKT1 (Met1-Ala480) 8xHis tag

Attributs du produit: Extracellular Domain Protein

Pureté: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1 (AKT1 Produits)

Synonymes: AKT Protein, CWS6 Protein, PKB Protein, PKB-ALPHA Protein, PRKBA Protein, RAC Protein, RAC-ALPHA Protein, Akt Protein, Rac Protein, PKB/Akt Protein, PKBalpha Protein, AKT/PKB Protein, AKT1 Protein, Akt/PKB Protein, Akt1 Protein, CG4006 Protein, D-Akt Protein, DAKT1 Protein, DAKT1/PKB Protein, DAkt Protein, DAkt1 Protein, DPKB Protein, DRAC-PK Protein, DRAC-PK66 Protein, DRAC-PK85 Protein, Dakt Protein, Dakt1 Protein, Dmel\\CG4006 Protein, Dpkb Protein, PKB/AKT Protein, PKB/dAKT Protein, RacPK Protein, akt Protein, akt1 Protein, dAKT Protein, dAKT/dPKB Protein, dAKT1 Protein, dAkt Protein, dAkt/PKB Protein, dAkt1 Protein, dPKB Protein, dakt Protein, dakt1 Protein, l(3)04226 Protein, l(3)89Bq Protein, p-Akt Protein, pAkt Protein, ACT-5 Protein, akt-1 Protein, pkb Protein, v-akt Protein, v-akt1 Protein, xAct Protein, ATAKT1 Protein, F18A8.2 Protein, F18A8_2 Protein, K+ transporter 1 Protein, POTASSIUM TRANSPORTER Protein, AKT serine/threonine kinase 1 Protein, thymoma viral proto-oncogene 1 Protein, CG4006 gene product from transcript CG4006-RE Protein, actin beta Protein, v-akt murine thymoma viral oncogene homolog 1 S homeolog Protein, v-akt murine thymoma viral oncogene homolog 1 Protein, K+ transporter 1 Protein, Serine/threonine-protein kinase akt-1 Protein, AKT1 Protein, Akt1 Protein, ACTB Protein, akt1.S Protein, akt1 Protein, KT1 Protein, akt-1 Protein

Sujet: AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Description: This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]

Poids moléculaire: predicted molecular mass of 56.8 kDa after removal of the signal peptide. The apparent molecular mass of AKT1-8xHis is 55-70 kDa due to glycosylation.

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg