NFKB1 anticorps (AA 740-964)
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- Antigène Voir toutes NFKB1 Anticorps
- NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
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Épitope
- AA 740-964
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NFKB1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Séquence
- AAGADPLVEN FEPLYDLDDS WENAGEDEGV VPGTTPLDMA TSWQVFDILN GKPYEPEFTS DDLLAQGDMK QLAEDVKLQL YKLLEIPDPD KNWATLAQKL GLGILNNAFR LSPAPSKTLM DNYEVSGGTV RELVEALRQM GYTEAIEVIQ AASSPVKTTS QAHSLPLSPA STRQQIDELR DSDSVCDSGV ETSFRKLSFT ESLTSGASLL TLNKMPHDYG QEGPL
- Réactivité croisée
- Humain, Souris, Rat
- Attributs du produit
- Polyclonal Antibodies
- Immunogène
- Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
- Isotype
- IgG
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- Indications d'application
- WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:100
- Commentaires
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HIGH QUALITY
- Restrictions
- For Research Use only
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- by
- Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum
- No.
- #104072
- Date
- 21.11.2019
- Antigène
- NFKB1
- Numéro du lot
- 3560449004
- Application validée
- Immunofluorescence
- Contrôle positif
U-937 human monocytic cell line
- Contrôle négative
No primary antibody control
- Conclusion
Passed. ABIN6144571 specifically stains NF-κB1 in U-937 human monocytic cells.
- Anticorps primaire
- ABIN6144571
- Anticorps secondaire
- TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.)
- Full Protocol
- Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO2 in 300µL in 24-well plates (Sarstedt AG&Co. KG).
- Use cytospin to spin cells onto slide.
- Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
- Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
- Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
- Wash cells 3x for 5min with PBS.
- Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
- Incubate cells with primary antibody rabbit anti-human NF-κ1/p50 (antibodies-Online, ABIN6144571, lot 3560449004) diluted 1:100 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
- Wash cells 3x for 5min with PBS.
- Incubate cells with secondary TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
- Wash cells 2x for 5min with PBS.
- Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
- Image acquisition with an Olympus widefield microscope.
- Notes
Strong red staining dependent on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.
Validation #104072 (Immunofluorescence)Validation ImagesProtocole -
- Format
- Liquid
- Buffer
- PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C. Avoid freeze / thaw cycles.
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Toll-Like Receptor 4 Mediates Methamphetamine-Induced Neuroinflammation through Caspase-11 Signaling Pathway in Astrocytes." dans: Frontiers in molecular neuroscience, Vol. 10, pp. 409, (2017) (PubMed).
: "Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB." dans: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).
: "
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Toll-Like Receptor 4 Mediates Methamphetamine-Induced Neuroinflammation through Caspase-11 Signaling Pathway in Astrocytes." dans: Frontiers in molecular neuroscience, Vol. 10, pp. 409, (2017) (PubMed).
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- Antigène
- NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
- Autre désignation
- NFKB1 (NFKB1 Produits)
- Synonymes
- anticorps GKLP, anticorps NKTL, anticorps NTKL, anticorps P105, anticorps TAPK, anticorps TEIF, anticorps TRAP, anticorps EBP-1, anticorps KBF1, anticorps NF-kB1, anticorps NF-kappa-B, anticorps NF-kappaB, anticorps NFKB-p105, anticorps NFKB-p50, anticorps NFkappaB, anticorps p105, anticorps p50, anticorps NF-kB, anticorps NF-KB1, anticorps ebp-1, anticorps kbf1, anticorps nf-kappa-b, anticorps nf-kappaB, anticorps nfkb-p105, anticorps nfkb-p50, anticorps NF-kappaB1, anticorps p50/p105, anticorps BICDR-1, anticorps bicdr1, anticorps ccdc64, anticorps SCY1 like pseudokinase 1, anticorps nuclear factor kappa B subunit 1, anticorps nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 L homeolog, anticorps nuclear factor NF-kappa-B p105 subunit, anticorps nucleoporin 107, anticorps nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105, anticorps nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, anticorps SCYL1, anticorps NFKB1, anticorps Nfkb1, anticorps nfkb1.L, anticorps CpipJ_CPIJ012236, anticorps Nup107, anticorps nfkb1
- Sujet
- This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.,NFKB1,CVID12,EBP-1,KBF1,NF-kB1,NF-kappa-B,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Signal Transduction,Cell Biology & Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology & Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,IL-6 Receptor Signaling Pathway,NF-kB Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,NFKB1
- Poids moléculaire
- 85 kDa/105 kDa
- ID gène
- 4790
- UniProt
- P19838
- Pathways
- Signalisation p53, Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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