This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-468 amino acids from the C-terminal region of human AKT1.
AKT1
Reactivité: Humain
WB, IHC, IF, IP
Hôte: Lapin
Monoclonal
unconjugated
Indications d'application
WB: 1:1000. WB: 1:1000. WB: 1:1000. FC: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Miao, Zheng, Li, Su, Zhao, Zhou, Jia: "MicroRNA-130b targets PTEN to mediate drug resistance and proliferation of breast cancer cells via the PI3K/Akt signaling pathway." dans: Scientific reports, Vol. 7, pp. 41942, (2017) (PubMed).
Li, Jia, Ren, Liu, Gong, Wang, Zhang, Zhao: "Axl mediates tumor invasion and chemosensitivity through PI3K/Akt signaling pathway and is transcriptionally regulated by slug in breast carcinoma." dans: IUBMB life, Vol. 66, Issue 7, pp. 507-18, (2014) (PubMed).
Cai, Wang, Li, Ayala, Ittmann, Liu: "GGAP2/PIKE-a directly activates both the Akt and nuclear factor-kappaB pathways and promotes prostate cancer progression." dans: Cancer research, Vol. 69, Issue 3, pp. 819-27, (2009) (PubMed).
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.